Fig. 11.

Alignment of exon-intron borders after insertion of intron 85c into exon 5. Intron 85c is found in ARR0 of bat star (Pmi) and vase tunicate (Cin), but not in acorn worm (Sko) or lancelet (Bfl) (highlighted in grey). Exon 5 of one of the non-visual arrestins in lampreys (shown: Lca) as well as in ARRB2 in all Euteleosteomorpha (Gmo, Gac, Ola, Oni, Tru, Xma) is split into two parts, denoted as 5.1 and 5.2. In contrast, exon 5 of ARRB2 is not split in Otomorpha (Dre, Ame) and spotted gar (Lco) (grey). Only the 5’- and 3’-parts of the intron sequences are shown (green box), while the larger inner region is left out being non-informative (black lines). The proto-splice site motif ‘AGGY’ is conserved for all species genes shown except for Otomorpha (‘AAGC’). The alignment was visualized with Jalview 2.8.1 [93]