Fig. 7.

S. spinosum extracts induced insulin secretion in Min6 cells. Min6 pancreatic beta-cells were grown as described and treated for 1 h with leaves (a and b) or fruits (c and d) S. spinosum extracts at various concentrations as indicated. Supernatant was collected at the basal state and after glucose induction of insulin secretion. The induction of insulin secretion and measurement of insulin secretion were performed as described in the Materials and Methods section. Results are mean ± SEM of five independent experiments, **p < 0.005, ***p < 0.0005 compared to untreated cells by Student’s t-test