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. 2017 Mar 20;7(2):e1308900. doi: 10.1080/21592799.2017.1308900

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Figure 4. — Golgi and PM localization of exogenously expressed GBF1 in GBM cells person D54 (A and B) and U87 (C and D) cells were transfected with GFP-tagged GBF1 and probed by double label IF with polyclonal anti-GFP and either monoclonal anti-GM130 (A and C) or monoclonal anti-paxillin antibodies (B and D). Exogenous GFP-GFP1 co-localizes with GM130 at the Golgi and co-localizes with paxillin at tips of cellular protrusions and the leading edge (arrows). Representative images from more than 3 independent experiments. Bar is 10 μm.