Table 1.

Promoter SNP genotype and methylation of MLH1 CpG island and shore in CRC cell lines. A panel of five colorectal carcinoma cell lines was genotyped by Sanger sequencing to determine genotype of rs1800734. MethyLight was utilized to determine the percentage of fully methylated alleles at the CpG island and shore of MLH1. Average PMR (percent methylated reference) of two duplicate reactions is shown.

Cell Line	rs1800734 Genotype	CpG shore PMR	CpG island PMR
HCT 116	GG	99.6%	0%
LS 174T	GG	73.1%	0%
SNU-C2B	GA	28.9%	0%
COLO 320HSR	GA	23.4%	0%
HCT-15	AA	34.6%	0%