Table 2.
Proline content (μmol g−1FW), glutamyl kinase (GK; U mg−1protein), proline oxidase (U mg−1protein) and pyrroline-5-carboxylate synthetase activity (P5CS; U min−1 mg−1protein) in mustard (Brassica juncea L.) cv Pusa Jai Kisan at 30 DAS. Plants were treated either with 5 mM N, 10 mM N, 200 µl l−1 ethephon in different combinations and grown with or without 100 mM NaCl. Data means with the same letter are not different significantly at P < 0.05. DW, dry weight; FW, fresh weight.
| Treatments | Proline content | GK activity | Proline oxidase | P5CS activity |
|---|---|---|---|---|
| Control | 5.9 ± 0.19h | 0.64 ± 0.0199h | 0.0883 ± 5.5000e-3a | 8.2 ± 0.246h |
| 5 mM N (N5) | 6.7 ± 0.21 g | 0.69 ± 0.022 g | 0.078 ± 3.8000e-3b | 9.1 ± 0.318 g |
| 10 mM N (N10) | 15.4 ± 0.71c | 1.55 ± 0.06c | 0.03 ± 1.9000e-3 g | 16.8 ± 0.84c |
| 100 mM NaCl | 14.0 ± 0.67f | 1.11 ± 0.037f | 0.066 ± 2.9000e-3c | 12.4 ± 0.49f |
| 200 µl l−1 ethephon (E) | 16.1 ± 0.79d | 1.4 ± 0.0613d | 0.0323 ± 2.0000e-3f | 15.6 ± 0.81d |
| N5 + NaCl | 17.6 ± 0.94e | 1.2200 ± 0.04e | 0.051 ± 2.4000e-3d | 14.0 ± 0.63e |
| N10 + NaCl | 12.1 ± 0.61d | 1.34 ± 0.056d | 0.044 ± 2.1000e-3e | 15.2 ± 0.745d |
| NaCl + E | 13.0 ± 0.62d | 1.36 ± 0.0572d | 0.041 ± 2.2000e-3e | 15.4 ± 0.748d |
| N5 + E | 13.9 ± 0.64b | 1.61 ± 0.071b | 0.027 ± 1.3000e-3h | 17.6 ± 0.968b |
| N10 + E | 14.2 ± 0.65a | 1.73 ± 0.083a | 0.021 ± 1.1000e-3 g | 18.7 ± 1.06a |
| N5 + NaCl + E | 15.0 ± 0.731c | 1.51 ± 0.0711c | 0.029 ± 1.6000e-3 g | 16.3 ± 0.848c |
| N10 + NaCl + E | 16.0 ± 0.88b | 1.64 ± 0.0741b | 0.022 ± 1.1000e-3i | 17.2 ± 0.95b |