Table 3.
Activity of superoxide dismutase (SOD; U mg−1 protein), ascorbate peroxidase (APX, U mg−1 protein) and glutathione reductase (GR; U mg−1 protein), reduced glutathione content (GSH; nmol g−1 fresh mass) and redox state (GSH/GSSG) in mustard (Brassica juncea L.) cv Pusa Jai Kisan at 30 DAS. Plants were treated either with 5 mM N, 10 mM N, 200 µl l−1 ethephon in different combinations and grown with or without 100 mM NaCl. Data means with the same letter are not different significantly at P < 0.05. DW, dry weight; FW, fresh weight.
| Treatments | SOD | APX | GR | GSH | GSH/GSSG |
|---|---|---|---|---|---|
| Control | 8.7 ± 0.81i | 1.1 ± 0.04j | 0.17 ± 0.01j | 310 ± 15.4i | 17 ± 0.78i |
| 5 mM N (N5) | 9.6 ± 0.86h | 1.3 ± 0.04i | 0.2 ± 0.01i | 372 ± 16.3 g | 20.6 ± 1.03h |
| 10 mM N (N10) | 11.4 ± 0.88f | 1.6 ± 0.09 g | 0.26 ± 0.02 g | 463 ± 18.4e | 26.1 ± 1.69e |
| 100 mM NaCl | 13 ± 0.92e | 2.1 ± 0.08f | 0.3 ± 0.02f | 355 ± 16.4h | 14.8 ± 0.59j |
| 200 µl l−1 ethephon (E) | 10.2 ± 0.82 g | 1.5 ± 0.07h | 0.23 ± 0.02h | 413 ± 18.0f | 24.0 ± 1.44f |
| N5 + NaCl | 14.3 ± 0.98d | 2.4 ± 0.09d | 0.33 ± 0.01e | 410 ± 17.6f | 17.8 ± 0.83i |
| N10 + NaCl | 15.7 ± 1.07c | 2.8 ± 1.11c | 0.44 ± 0.02c | 487 ± 19.5d | 23.1 ± 1.27 g |
| NaCl + E | 16.0 ± 1.1c | 3.0 ± 0.14c | 0.46 ± 0.02c | 490 ± 20.2d | 22.6 ± 1.13 g |
| N5 + E | 14.4 ± 0.99d | 2.3 ± 0.12e | 0.31 ± 0.02e | 494 ± 19.9d | 30.3 ± 2.12b |
| N10 + E | 16.3 ± 1.18c | 2.9 ± 0.14c | 0.36 ± 0.03d | 579 ± 26.3a | 37.6 ± 3.00a |
| N5 + NaCl + E | 19.0 ± 1.23b | 3.3 ± 0.17b | 0.50 ± 0.024b | 527 ± 23.0c | 27.7 ± 1.66d |
| N10 + NaCl + E | 21.6 ± 1.36a | 3.7 ± 0.20a | 0.54 ± 0.029a | 553 ± 25.2b | 29.9 ± 2.09c |