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. Author manuscript; available in PMC: 2018 May 31.
Published in final edited form as: Vaccine. 2017 May 4;35(24):3232–3238. doi: 10.1016/j.vaccine.2017.04.067

Figure 1. Characterization of Pfs25-EPA conjugate desorbed from Alhydrogel formulation following Forced Degradation Study.

Figure 1

A) SDS-PAGE (silver staining); B) Western blot: (a) probed with monoclonal antibody 4B7; (b) probed with penta-His; (c) probed with anti-Exotoxin A. Lane 1, Pfs25-EPA reference standard (without desorption); Lane 2, Desorption of Pfs25-EPA/Alhydrogel stored at 4°C for 11 days, Lane 3, Desorption of Pfs25-EPA/Alhydrogel stored at 60°C for 11 days. All samples were loaded at 550 ng protein (calculation based on 100% recovery of desorption) per lane. Molecular weight markers are indicated in kDa.