Figure 1. Workflow of the LDHr assay.

The LDHr assay follows a straightforward three-step protocol: 1.) addition of test compounds (e.g. tBHP); 2.) LDHr assay by transfer of a 50 μl sample of tissue culture supernatant to the Assay Plate and addition of 50 μl Assay Buffer; 3.) addition of 50 μl Stop Solution following a one hour incubation in the dark and subsequent measurement of absorbance at λ = 490 nm (OD₄₉₀).