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. Author manuscript; available in PMC: 2018 May 2.
Published in final edited form as: Curr Protoc Toxicol. 2017 May 2;72:2.26.1–2.26.10. doi: 10.1002/cptx.21

Figure 3. Sample results for Trolox-mediated cytoprotection using the LDHr assay.

Figure 3

A.) Trolox protects primary cultured E18 rat cortical neurons from tBHP-induced oxidative stress, by shifting the EC50 from 26.2 μM to 37.1 μM. B.) Analogously, Trolox protects primary cultured adult rat optic nerve head astrocytes from tBHP-induced oxidative stress, by shifting the EC50 from 264.5 μM to 419.1 μM. For both experiments, data was plotted in Prism 7.0 software (GraphPad, La Jolla, CA) and analyzed by non-linear regression using a four-parameter logistic equation with variable Hill slope, performed on the mean value of the technical replicates (n = 4) to determine the mean EC50 values for tBHP for each pre-treatment condition (Control vs. Trolox).