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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Apr 28;1862(8):731–739. doi: 10.1016/j.bbalip.2017.04.006

Figure 4. Selected Reaction Monitoring of the total ion current chromatograms of targeted oxidized aliphatic chains.

Figure 4

The carboxyl linkage of targeted oxidized aliphatic chains were charge-switch derivatized with aminomethyl phenyl pyridium (AMPP) by condensation with carbodiimide. The resultant charge-switch derivatized oxidized metabolites of linoleic (LA), arachidonic (AA) and docosahexaneoic (DHA) acids possessed ~30-80 fold increases in S/N due both to the switch to the positive ion mode in conjunction with the permanent positive charge in the pyridinium ring. The indicated fatty acid metabolite standards were derivatized with AMPP and measured by LC MS/MS via SRM in the positive ion mode following chromatographic separation using a C18 reverse phase HPLC column. Signature transitions from each of the analytes were used (as shown) and product ions were collected and reinjected into the Orbitrap™ to further substantiate the integrity of the SRM through accurate mass determination of their molecular identity. Representative SRM chromatograms for the standards are shown whose product ions contained the anticipated masses after reinjection into the Orbitrap™.

(Anal. Biochem. 2013 Jul11; 442:40-50. http://dx.doi.org/10.1016/j.ab.2013.06.014 2005 American Chemical Society)