(A) Tactile responses to von Frey filaments for HFD mice receiving saline (HFD saline), insulin at 0.5U (HFD 0.5U insulin) or insulin at 2.0U (HFD 2.0U insulin). Two-way repeated measures ANOVA followed by Sidak’s post-hoc test, *p<0.05 vs baseline, n=8. (B) Blood glucose levels in HFD mice receiving saline (HFD saline), insulin at 0.5U (HFD 0.5U insulin) or insulin at 2.0U (HFD 2U insulin). Two-way ANOVA, **p<0.01, ***p<0.001 vs baseline followed by Tuley’s post hoc test, n=8. Mean+s.e.m.