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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Cancer Res. 2017 Feb 15;77(7):1753–1762. doi: 10.1158/0008-5472.CAN-16-2374

Figure 1. Chromatin regulator focused RNAi screen in MLL-AF9 leukemia identifies Mof as a key regulator.

Figure 1

(A) Schematic outline of chromatin regulator focused shRNA library screen coupled with high-throughput sequencing (HiSeq) in murine primary bone marrow (BM) MA9 monoclonal leukemia cells.

(B) Volcano plot depicting the changes in representation (x-axis) and significance (y-axis) of each shRNA construct in the screen before versus 12 days after hairpin-induced knockdown. One dot represents the mean for two independent experiments. The dotted area contains the 20 most significantly depleted hairpins in the screen (more than 256-fold depletion, significance (defined as −log10 of the p-value) ≥ 4.5). Total library (gray; 2,252 shRNAs, 468 genes), shMof (red; four shRNAs) and shBRD4 (blue; five shRNAs) are highlighted as the only two genes with two hits.

G418: Neomycin; Dox: Doxycycline.