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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Cancer Res. 2017 Feb 15;77(7):1753–1762. doi: 10.1158/0008-5472.CAN-16-2374

Figure 4. Homozygous Mof loss in MLL-AF9 transformed mouse LSKs leads to cell death and DNA damage.

Figure 4

(A) MA9 in vitro transformed Moff/f or Mof+/+ LSKs were infected with full-length Mof or empty vector control (miCD2) and selected by sorting hCD2 positive cells. Cells were then plated in liquid culture immediately upon sorting dTomato positive cells, 48 hours after infection with dTomato-Cre. PCR analysis illustrates excision at 48, 72, 96 and 144 hours post Cre infection.

(B) Gene ontology analysis with a differential expression analysis list of significantly (p < 0.05) downregulated genes comparing Moff/f cells to Mof+/+ cells at 72 hours post Cre infection.

(C) Number of large yH2AX foci per cell nucleus at 48 or 72 hours post Cre infection. A dot represents a single cell.

** p < 0.01. Error bars represent SD of mean.