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. 2017 Jul 7;12(7):e0179835. doi: 10.1371/journal.pone.0179835

Fig 4. Induction of myocyte autophagy by Ang II and STAT3 inhibition.

Fig 4

(A) GFP-LC3 puncta (green) in H9c2 cells transfected with GFP-LC3 and treated with vehicle (Control) or Ang II and WP1066 alone or in combination. Scale bar = 10 μm. (B) Quantitation of GFP-LC3 autophagosomes (green puncta) in H9c2 cells treated with vehicle (Control) or Ang II and WP1066 alone or in combination. (C) Protein expression of autophagy markers p62 and LC3 in H9c2 cells treated with Ang II or/and WP1066. (D) Densitometric analysis of p62 and LC3 protein expression levels. Data represent mean ± SEM (n = 3). *P<0.05 vs. control; #P<0.05 vs. Ang II only.