Fig 1. Preconditioning with CoCl₂ is more effective than intermittent clamping (IC).

(A) Experimental design for the in vivo study comparing the ability of different preconditioning techniques to protect against IRI in rats. (B) Animal sickness scores in rats treated with saline (control) (●), 30 mg/kg CoCl₂ (■), IC (⬢) or IC + CoCl₂ (▲). (C) Serum creatinine concentrations (mean ± SEM (µmol/L)). The rise in serum creatinine was less in rats preconditioned with 30 mg/kg CoCl₂ than in the saline control group. (D) Serum urea concentrations (mean ± SEM (mmol/L)). *p<0.05 Vs saline treated control.