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. 2017 Jul 7;12(7):e0180393. doi: 10.1371/journal.pone.0180393

Fig 2. Gemigliptin attenuates high phosphate-induced vascular calcification in VSMCs.

Fig 2

(A) Visualization of calcium deposition on VSMCs was assessed by alizarin red staining. Representative pictures were shown. (B) After HCl decalcification, calcium content of the cells was assayed. The value was normalized by the total protein and expressed as percentage of control. (C) Cell viability was determined using MTT assay. The results are expressed as percentage of control. Results are presented as mean ± SEM (n = 6–7 in each group). Con, control; Gemi50, 50 μM gemigliptin; Pi, 3mM phosphate; PG50, 3 mM phosphate and 50 μM gemigliptin. ***P<0.001 compared with control and ###P<0.001 compared with phosphate group.