Table 4. Summary of testing of NPW specimens collected during phase II LAIV trial in Bangladesh (samples collected on days 2, 4 and 7 after vaccination).
| Study group | Type | Subtype/ lineage |
NPW specimens by qRT-PCR (n = 287) |
NPW specimens by sequencing (n = 2843) | ||||
|---|---|---|---|---|---|---|---|---|
| N positive1 | Ct value range | N positive4 | Vaccine-type | Wild-type | Inconclusive | |||
| LAIV | A | H1N1pdm | 1 | 38.25 | 0 | - | - | - |
| H3N2 | 138 | 21.90–39.96 | 134 | 133 | 0 | 1 | ||
| Unsubtyped | 0 | - | 65 | 6 | 0 | 0 | ||
| B | Yamagata | 1962 | 20.27–41.18 | 0 | - | - | - | |
| Victoria | 223 | 221 | 1 | 1 | ||||
| Placebo | A | H1N1pdm | 0 | - | 0 | - | - | - |
| H3N2 | 0 | - | 0 | - | - | - | ||
| Unsubtyped | 0 | - | 0 | - | - | - | ||
| B | Yamagata | 72 | 20.90–38.86 | 6 | 0 | 6 | 0 | |
| Victoria | 1 | 1 | 0 | 0 | ||||
1co-infection with two viruses was detected in 55 subjects
2qRT-PCR protocol did not distinguish influenza B lineages
3Three NPW specimens positive by qRT- PCR (Ct values 34.50–42.66) were negative by RT-PCR
4co-infection with two or three viruses was detected in 86 subjects
5positive for H3/HA by qRT-PCR (Ct values 35.71–39.96).