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. 2017 Jul 7;12(7):e0180786. doi: 10.1371/journal.pone.0180786

Fig 2. hEphA2 expression in tumor cells.

Fig 2

(A) Western blot analysis of hEphA2. Lysates of PC3 and HeLa cells (8 × 105) were separated by SDS-PAGE and transferred to nylon membranes. Membranes were stained with rabbit anti-hEphA2 or β-actin antibodies. (B) Flow cytometric analysis of cells. Cells (5 × 105) were stained with mouse anti-hEphA2 and Alexa 488-conjugated anti-mouse IgG antibodies (anti-hEphA2), and fluorescence was measured by flow cytometry. Unstained cells or cells stained with Alexa 488-conjugated anti-mouse IgG antibody (2nd Ab) alone were used as controls.