Skip to main content
. 2017 Jul 3;11:1989–2006. doi: 10.2147/DDDT.S136792

Figure 4.

Figure 4

Sal pretreatment inhibits the production of IL-6 and TNF-α in hepatic IRI.

Notes: (A) The serum IL-6 and TNF-α levels were measured by ELISA and expressed as mean ± SD at 2, 8, and 24 hours after reperfusion in mice. Serum levels of IL-6 and TNF-α were significantly increased in IRI mice. Sal pretreatment dramatically reduced the serum levels of IL-6 and TNF-α, particularly at 20 mg/kg. (B) The relative mRNA levels of IL-6 and TNF-α were evaluated in each group, as shown by qRT-PCR. (C) Protein expression of IL-6 and TNF-α was detected by Western blot. Data were given as mean ± SD (n=6, *P<0.05 for Sham versus IRI, #P<0.05 for IRI + Sal [10 mg/kg] versus IRI, and †P<0.05 for IRI + Sal [20 mg/kg] versus IRI + Sal [10 mg/kg]).

Abbreviations: IL-6, interleukin-6; IRI, ischemia–reperfusion injury; Sal, salidroside; TNF-α, tumor necrosis factor-α; qRT-PCR, quantitative real-time polymerase chain reaction.