Table 1.
Primers used for qRT-PCR in this study
| Gene | GenBank accession number | Primers sequence (5′-3′) |
|---|---|---|
| β-actin | NM_007393 | |
| Forward | GGCTGTATTCCCCTCCATCG | |
| Reverse | CCAGTTGGTAACAATGCCATGT | |
| IL-6 | NM_031158 | |
| Forward | CTGCAAGAGACTTCCATCCAG | |
| Reverse | AGTGGTATAGACAGGTCTGTTGG | |
| TNF-α | NM_013693 | |
| Forward | CAGGCGGTGCCTATGTCTC | |
| Reverse | CGATCACCCCGAAGTTCAGTAG | |
| BcI-2 | NM_177410 | |
| Forward | GCTACCGTCGTCGTGACTTCGC | |
| Reverse | CCCCACCGAACTCAAAGAAGG | |
| Bax | NM_007527 | |
| Forward | AGACAGGGGCCTTTTTGCTAC | |
| Reverse | AATTCGCCGGAGACACTCG | |
| Caspase 3 | NM_009810 | |
| Forward | CTCGCTCTGGTACGGATGTG | |
| Reverse | TCCCATAAATGACCCCTTCATCA | |
| Caspase 9 | NM_015733 | |
| Forward | GGCTGTTAAACCCCTAGACCA | |
| Reverse | TGACGGGTCCAGCTTCACTA | |
| Beclin-1 | NM_019584 | |
| Forward | ATGGAGGGGTCTAAGGCGTC | |
| Reverse | TGGGCTGTGGTAAGTAATGGA | |
| LC3 | NM_026160 | |
| Forward | GACCGCTGTAAGGAGGTGC | |
| Reverse | AGAAGCCGAAGGTTTCTTGGG | |
| P62 | NM_011018 | |
| Forward | GAGGCACCCCGAAACATGG | |
| Reverse | ACTTATAGCGAGTTCCCACCA |
Abbreviations: IL-6, interleukin-6; LC3, light chain 3; qRT-PCR, quantitative real-time polymerase chain reaction; TNF-α, tumor necrosis factor-α.