Fig 5. Leishmania donovani containing PV specifically recruits Rab5a human PBMC differentiated macrophages.

A. Levels of Rab5a in infected and uninfected PBMC differentiated macrophages after 24 h of infection were determined by qPCR as described in Materials and Methods. B. Human PBMC differentiated macrophages were infected with Leishmania (MOI 1:20) and level of expression of miR-494 in uninfected and infected cells was determined at indicated time by qPCR as described in Materials and Methods. All results are represented as mean ± S.D. of three independent experiments and normalized to respective control. Expression of normalized respective data in uninfected cells was arbitrarily chosen as one unit. C. Human PBMC differentiated macrophages were infected with Leishmania and recruitment of Rab5a was determined after 24 h of infection by immuno-staining with specific antibody as described in Materials and Methods. Uninfected cells were used as control. Results are representative of three independent experiments.