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. 2017 Jun 26;11(6):e0005675. doi: 10.1371/journal.pntd.0005675

Fig 3. Sustained IL-4Rα mediated signaling is critical for the anti-parasitic type 2 immune responses during primary N. brasiliensis infection.

Fig 3

Mice were infected with 500 L3 N. brasiliensis, fed with tamoxifen once daily from day 5 to day 8 post infection and killed 9 days post-infection. A. Schematic showing the experimental design. B. IL-4Rα expression on CD3+CD4+ T-cells from MLN of N. brasiliensis-infected mice. C. Cytokine production detected by ELISA in the supernatant of restimulated MLN cells. D. Percentages of cytokine-producing CD4+ T-cells after stimulation with PMA/Ionomycin/Monensin cocktail. E. Percentages of transcription factor-expressing CD3+CD4+ T-cells (ex-vivo) summarized in (F). G. Total serum IgE in N. brasiliensis-infected mice. H. NbAg-specific serum type 2 antibodies. I. Eosinophil (SiglecFhi) numbers per MLN. J. Worm counts per gut. Each experiment was conducted at least twice with 4–12 mice per group. Data are expressed as mean ± SD; NS = p > 0.05; * = p < 0.05; ** = p < 0.01; *** =, p < 0.001; **** = p < 0.0001.