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. 2017 Jun 26;11(6):e0005675. doi: 10.1371/journal.pntd.0005675

Fig 4. Sustained IL-4Rα mediated signaling is required for recall responses to re-infection with N. brasiliensis.

Fig 4

Mice were infected with 500 L3 N. brasiliensis, treated with Ivermectin as from day 9 post infection, fed with tamoxifen once daily from day 31 to day 34 then re-infected with 500 L3 N. brasiliensis at day 37 and killed 5 days post-infection. A. Schematic showing the experimental design. B. IL-4Rα expression on mdLN CD3+CD4+ T cells after secondary infection with N. brasiliensis. C. Worm counts per gut. D. PAS staining of pulmonary mucus producing goblet cells. E. Total numbers of various CD3+CD4+ T cell subsets (ex-vivo). F. Percentages and total numbers of Gata-3+ CD4+ T-cells. G. Cytokine release detected by ELISA in the supernatant of mediastinal lymph node cells either left unstimulated primed with the parasite antigen NbAg or restimulated with anti-CD3 antibodies. H. Percentages of cytokine-producing mediastinal lymph nodes CD4+ T-cells after stimulation with PMA/Ionomycin/Monensin cocktail. I. Total ILC2 numbers in mediastinal lymph nodes. Each experiment was conducted at least twice with 4–5 mice per group. Data are expressed as mean ± SD; * = p < 0.05; ** = p < 0.01.