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. 2017 Jun 26;13(6):e1006437. doi: 10.1371/journal.ppat.1006437

Fig 4. Neutrophils do not contribute significantly to infection.

Fig 4

(A) Embryo survival (left; representative experiment) and bacterial burden (total of 2 experiments) over time (right panel, geometric mean) of control (black) and gp91 knockdown embryos (red) iv injected with B. stabilis. Each data point represents an individual embryo. (B) Embryo survival of mpx/umn+ embryos, untreated or treated with 10 mM Mtz or 0.2% DMSO, and mpx/umn−embryos treated with 10 mM Mtz iv injected with ~ 50 CFU B. cenocepacia K56-2. (C) Embryo survival (average inoculum 50 CFU, representative experiment) of control (black) and csf3R knockdown embryos (red) injected iv with B. cenocepacia K56-2. * p ≤ 0.05; ** p ≤ 0.01; ns: non-significant. See materials and methods for statistical tests.