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. 2017 Jul 3;23:372–384.

Figure 5.

Figure 5

PN-1 protein in the bovine retina. Bovine interphotoreceptor matrix (IPM) lavage and RPE and retina lysates were prepared. Protein samples were resolved in a polyacrylamide gel, transferred to nitrocellulose membranes, and immunostained with Ab-PN-1 (A) or Ab-PEDF (B). Photos of the blots with immunostaining are shown as indicated at the bottom. A: The samples were loaded as follows: fetal bovine serum (FBS; 10 μl of 10% FBS, lane 1), lavage of bovine IPM with PBS (15 μg, lane 2), lavage of bovine IPM with 1 M NaCl in PBS (20 μg, lane 3), bovine RPE lysates (10 μg, lane 4), and bovine retina lysate (20 μg, lane 5). B: The samples were loaded as follows: FBS (10 μl of 10% FBS, lane 1), lavage of bovine IPM with 1 M NaCl in PBS (20 μg, lane 2), lavage of bovine IPM with PBS (15 μg, lane 3), bovine RPE lysates (10 μg, lane 4), and bovine retina lysates (20 μg, lane 5). For each panel, the migration positions of the MW markers are shown to the left. The migration positions of PN-1 and PEDF are shown by * and **, respectively, to the right of each blot.