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. 2017 Jun 26;13(6):e1006451. doi: 10.1371/journal.ppat.1006451

Fig 8. HERV-K Env activates the ERK1/2 MAPK pathway upstream of Raf.

Fig 8

(A) The ERK1/2 pathway was targeted by different chemical inhibitors: FTI-277 (a H/K-Ras inhibitor), TAK 632 (a pan Raf inhibitor), and U0126 (a broadly-used antagonist of MEK1/2). (B) The experimental procedure is illustrated on the scheme. Briefly, 293T cells were transfected with the indicated plasmids. Inhibitors (or vehicle control) were added when media was changed 18 hours post-transfection. Lysates and total RNA were harvested 48 hours post transfection. Activation of ERK1/2 (C) and induction of transcription factor expression (D) were assayed as described previously (Figs 2C & 3B) in the presence (+) or absence (-) of FTI-277, TAK 632 or U0126. Error bars represent the standard deviation of the mean of five independent experiments.