Key Resources Tables.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Guinea pig anti-Insulin | DAKO | A0564 RRID:AB_10013624 |
| Rabbit anti-Ki67 | Lab Vision Corp | RM-9106-S0 RRID:AB_149919 |
| Rat anti-BrdU | Novus Biologicals | NB500-169 RRID:AB_10002608 |
| Goat-anti-GFP | Abcam | ab13970 RRID:AB_300798 |
| Rabbit-anti-MafA | Bethyl Labs | A300-611A RRID:AB_2297116 |
| Rabbit-anti-Pdx1 | Abcam | AB47267 RRID:AB_777179 |
| Rabbit-anti-Nkx6.1 | LifeSpan BioSciences | LS-C143534 RRID:AB_10947571 |
| Mouse-anti-Glucagon | Sigma | G2654 AB_259852 |
| Mouse-anti-Somatostatin | BCBC | AB1985 RRID:AB_10014609 |
| Alexa-Fluor-647-conjugated insulin mAb | Cell Signaling Technology | 9008s |
| Chemical Reagents, Peptides, and Recombinant Proteins | ||
| Collagenase Type IV | Sigma | 639207 |
| Liberase TL | Roche | 05401020001 |
| Histopaque | Sigma | 10771 |
| Accumax | Life Technologies | AM105 |
| Accutase | Life Technologies | A1110501 |
| SYTOX® Blue Dead Cell Stain | Life Technologies | S34857 |
| TMRM | Life Technologies | T668 |
| MitoTracker Green | Life Technologies | M7514 |
| MitosoxRed | Life Technologies | M36008 |
| L-Proline | Sigma | P5607 |
| L-Serine | Sigma | S4311 |
| L-Lysine | Sigma | L5501 |
| L-Tysorine | Sigma | T8566 |
| FCCP | Thermo Fisher Scientific | NC0904863 |
| GS System GS Media Supplement | Millipore | GSS-1016-C |
| Critical Commercial Assays | ||
| C1 Single-Cell Auto Prep Reagent Kit for RNA-seq | Fluidigm | 100-6201 |
| SMARTer Ultra Low RNA kit for Illumina Sequencing | Clontech | 634833 |
| Click-iT EdU Alexa Fluor 488 imaging kit | Life Technologies | C10337 |
| iScript cDNA Synthesis Kit | BioRad | 1708891 |
| DNeasy Blood & Tissue Kit | QIAGEN | 69504 |
| RNeasy Micro Kit | QIAGEN | 74004 |
| LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells | Life Technologies | L3224 |
| ApopTag Red In Situ Apoptosis Kit | Thermo Fisher Scientific | S7165 |
| mouse insulin ELISA kit | Alpco | 80-INSHU-E10.1 |
| SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing | Clontech | 634889 |
| Nextera XT DNA Sample Preparation Kit | Illumina | FC-131-1096 |
| Nextera XT DNA Sample Preparation Index Kit | Illumina | FC-131-1002 |
| Advantage® 2 PCR Kit | Clontech | 639207 |
| Deposited Data | ||
| RNAseq data | This manuscript | GEO:GSE86479 |
| H3K27ac_ChIP | GEO: GSE68618 | GSM1677162 |
| H3K27ac_Input | GEO: GSE68618 | GSM1677164 |
| Experimental Models: Cell Lines | ||
| HEK293T cells | ATCC | |
| Experimental Models: Organisms/Strains | ||
| Mouse: RIP-Cre | The Jackson Laboratory | 003573 |
| Mouse: R26YFP | The Jackson Laboratory | 006148 |
| Mouse: C57BL/6 | Charles River Laboratories | 027 |
| Mouse: mIns1-H2B-mCherry | Benner et al., 2014 | |
| Mouse: C57BL/6.mCAT | Dai et al., 2011 | |
| Oligonucleotides | ||
| Primers for quantitative PCR | This manuscript | See table in quantitative PCR analysis section |
| Recombinant DNA | ||
| pLenti-C-mGFP | Origene | PS100071 |
| pLenti-C-mGFP-Srf | Origene | MR208120L2 |
| pCMV-R8.74 | Addgene | 22036 |
| pMD2.G | Addgene | 12259 |
| Software and Algorithms | ||
| FlowJo 8.7 software | https://www.flowjo.com/solutions/flowjo | N/A |
| ImageJ software | https://imagej.nih.gov/ij/ | N/A |
| Prism 5 software (GraphPad Software) | https://www.graphpad.com/scientific-software/prism/ | N/A |
| STAR | https://github.com/alexdobin/STAR | N/A |
| Cufflink and Cuffdiff | http://cole-trapnell-lab.github.io/cufflinks/ | N/A |
| Orange | http://orange.biolab.si/, https://bitbucket.org/biolab/orange-differentiation | N/A |
| Bioconductor | https://www.bioconductor.org/ | N/A |
| GSEA | http://www.broad.mit.edu/gsea | N/A |
| HOMER | http://homer.ucsd.edu/homer/ | N/A |
| STRING | http://string-db.org/ | N/A |
| Monocle | Trapnell et al., 2014 | http://cole-trapnell-lab.github.io/monocle-release/ |
| TSCAN | Ji et al., 2016 | https://github.com/zji90/TSCAN |
| Embeddr | Campbell et al., 2015 | https://github.com/kieranrcampbell/embeddr |
| DeLorean | Reid et al., 2016 | https://cran.r-project.org/web/packages/DeLorean/index.html |
| 1D Pseudotime Scale | This manuscript | See scripts in Data S1 |