The SPS system is required for amino acid utilization and environmental pH manipulation. Wild type SC5314 Candida albicans, ssy1Δ/Δ (CaPM07), ptr3Δ/Δ (CaPM15) and ssy5Δ/Δ (CaPM23), and complemented strains were grown at 37°C on yeast nitrogen base supplemented with 1% casamino acids, with an initial pH of 4.0. The stp2Δ/Δ (SVC17) strain was included for comparison purposes.
A. Growth was determined by measuring the OD600 at indicated time points.
B. Aliquots of the cultures were used to measure the pH of the medium at the same time points. Graphs show mean and standard deviation from three independent replicates.