Expression of a truncated STP2 allele lacking the N-terminal domain restores growth and neutralization ability in the SPS mutants. A truncated allele of STP2 lacking the first 100 residues (Stp2*), corresponding to the N-terminal nuclear exclusion domain, was expressed in the Candida albicans ssy1Δ/Δ (CaPM36), ptr3Δ/Δ (CaPM38) and ssy5Δ/Δ (CaPM40) strains. Cultures were grown at 37°C on yeast nitrogen base supplemented with 1% casamino acids, with an initial pH of 4.0.
A. Growth was determined by measuring the OD600 at indicated time points.
B. An aliquot of the cultures was used to measure the pH of the medium at the same time points. Graphs show mean and standard deviation from three independent replicates.