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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Cell Microbiol. 2016 May 27;18(11):1611–1624. doi: 10.1111/cmi.12600

Fig. 6.

Fig. 6

The SPS system is required for normal resistance towards macrophage attack and cytotoxicity. RAW264.7 murine macrophages were infected with wild type (SC5314), ssy1Δ/Δ (CaPM07), reconstituted (CaPM25) and STP2*-expressing (CaPM36) cells.

A. An end point dilution assay coupled with an XTT metabolic assay was performed to determine the sensitivity of these strains towards macrophage killing. Bars show mean and standard deviation of five independent experiments. **P < 0.01.

B. Macrophage cytotoxicity was determined by measuring the release of lactate dehydrogenase after coincubation of phagocytes with fungal cells for 15 h at two different multiplicities of infection (MOI). Bars show mean and standard deviation of three independent experiments. *P < 0.05 (compared with WT at MOI 2); #P < 0.05 (compared with WT at MOI 1).