Skip to main content
. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Cell Microbiol. 2016 May 27;18(11):1611–1624. doi: 10.1111/cmi.12600

Fig. 7.

Fig. 7

The SPS system is required for neutralization of the phagosome. LysoTracker Red (LTR)-loaded RAW264.7 macrophages were infected with SC5314 (CaPM57), stp2Δ/Δ (CaPM61), ssy1Δ/Δ (CaPM62) and ssy1Δ/Δ + SSY1 (CaPM66) each expressing a C-terminal Pma1-GFP fusion.

A. Quantification of the LTR signal was performed using Slidebook 6.0. GFP and LTR signals were measured by drawing a line traversing the fungal cell (dashed line).

B. Representative profiles from each strain. GFP signal is plotted on the left axis, LTR signal plotted on the right axis. Area under the curve (filled in red) was calculated in Prism (GraphPad Software).

C. Graph shows box and whiskers (minimum to maximum values), representing the area under the curve from the LTR intensity. A minimum of 30 cells were analyzed per strain. The experiment was performed in triplicate.