Figure 7. Egfr^Δmye mice demonstrate decreased pro-angiogenic chemokine/cytokine production and angiogenesis during colon tumorigenesis.

(a) mRNA levels of the pro-angiogenic chemokine, Cxcl1, and the pro-angiogenic cytokine, Vegfa, were assessed by qRT-PCR from colonic tissues 77 days post-AOM injection. n = 6-8 control tissues and 8-10 tumors with paired non-tumor area per genotype. (b) Protein levels of pro-angiogenic chemokine, CXCL1, and pro-angiogenic cytokine, VEGF, were assessed by Luminex Multiplex Array from colonic tissues 77 days post-AOM injection. n = 5 control tissues and 6-9 tumors with paired non-tumor area per genotype. In (a) and (b), *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA with Kruskal-Wallis test, followed by Mann-Whitney U test. (c) Representative images of CD31⁺ blood vessel immunohistochemistry in colonic tissues. Scale bars = 50 μm. (d) Quantification of the total number of CD31⁺ blood vessels in (c). n = 3 control colonic tissues and 4-5 AOM-DSS-treated colonic tissues per genotype. (e) Quantification of the total CD31⁺ blood vessel area within tissues in (c). n = 3 control colonic tissues and 4-5 AOM-DSS-treated colonic tissues per genotype. In (d) and (e), ***P < 0.001 by one-way ANOVA with Newman-Keuls post-test.