Figure 6.

ILC2s accumulate and produce IL-13 post-PNX. (A) Immunofluorescent stains on sections from lungs of IL5^RFP/+ (Red5) mice show ILC2s near small airways and in alveolar spaces post-PNX. Scale bar = 100 um. (B, C) Flow cytometry of dissociated lungs from Il5^RFP/+ mice shows increased numbers of CD45⁺Lin⁻Thy1⁺IL5⁺ ILC2s 7d post-PNX compared to sham operated animals. (D, E) Flow cytometry of dissociated lungs from Smart13 mice shows increased numbers of IL-13 producing ILC2s 4d post-PNX compared to sham operated animals. n≥3 animals for each group. Data are represented as mean +/− SD. (F) Proposed model demonstrating the roles of CCR2+ monocytes, M2-like macrophages, and ILC2s in PNX-induced lung regeneration. See also Figure S6.