Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 7;7:4880. doi: 10.1038/s41598-017-05033-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Constitutive activation of the NLRP3 inflammasome in osteochondro-progenitors does not cause inflammation and abnormal growth plate development. All data were obtained from 4-week old WT, Nlrp3 ^DM1, Ikk2 ^DM1 or Nlrp3 ^DM1; Ikk2 ^DM1 male mice (n = 3–10/genotype). (A) Red blood cell-depleted bone marrow cells were stained with isotype control (data not shown) or with antibodies against CD11b and Gr1. Representative flow cytometry dot plots of the CD11b⁺/Gr1⁺ myeloid cells from each genotype are shown. (B) The percentage of CD11b⁺/Gr1⁺ cells in bone marrow cells. Data are expressed as mean ± SEM. *P < 0.05; **P < 0.005. (C) IL-1β levels in conditioned media from BMM treated with 100 ng/ml LPS for 3 hours, then with 5 mM ATP for 30 minutes. Data are expressed as mean ± SD of 3 independent experiments carried out in triplicates. NS, not significant. (D) H&E staining. GP, growth plate. Scale bar, 100 µm. (E) 3D μCT reconstruction of distal femoral metaphysis. Scale bar, 1 mm.