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. 2017 Jul 7;7:4880. doi: 10.1038/s41598-017-05033-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activation of NLRP3 in myeloid cells impairs chondrocyte responses to hypoxia. (A) Femoral sections from 2-week-old WT or Nlrp3 ^LysM male mice injected with hydroxyprobe were stained with IgG or hydroxyprobe antibody. A specimen from renal medulla was used as a positive control. Staining is indicated by the brown color. HZ, hypertrophic zone. (B) qPCR analysis of mRNA isolated from mouse epiphyses. Quantitative data were obtained from 2–3 mice/genotype and expressed as the mean ± SEM. *P < 0.05; **P < 0.005.