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. 2017 Jul 7;7:4845. doi: 10.1038/s41598-017-04936-7

Figure 8.

Figure 8

The expression domains of hth and Hr46 are complementary and co-expression of hth + tsh repress Hr46. hth:YFP late L3 disc stained with anti-Hr46 (A) and the corresponding optical cross-section (A’). The arrow marks the morphogenetic furrow (MF) and the dashed line marks the boundary between Hth and Hr46 expression. Clones overexpressing hth (B,B’), tsh (C,C’) or both (D,D’), marked by GFP (and outlined with the red dashed line), were induced in the eye imaginal disc at 48–72 hours after egg laying. Discs are stained with anti-Hr46. (E–E”’) in situ hybridization with ftz-f1 anti-sense probe in third-instar eye discs from optix > GFP (E’), optix > ßfyz-f1 (E”) and optix > hth.tsh (E”’) larvae. ftz-f1 sense probe was used as a control in optix > GFP eye discs (black dashed line outlines the disc) (E). ftz-f1 is transcribed in control eye discs in a dynamic pattern, with high expression in the anterior region and lower levels in the most posterior region. Red dashed lines mark the region where ftz-f1 expression is expected to be higher in optix > ßfyz-f1 discs. hth + tsh discs show higher ftz-f1 levels (black arrowheads mark the regions with especially strong ftz-f1 transcription).