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. 2017 Jul 7;7:4852. doi: 10.1038/s41598-017-04941-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Blast-induced tinnitus (+) rats had higher neural activity in two amygdala subdivisions and in the nucleus accumbens core (NAc) compared to control (ctrl) rats, though there were no significant differences between tinnitus (+) and tinnitus (−) rats. Images were color-coded according to manganese uptake intensity, as defined by the color scale-bar. Color-coded images are shown for a representative tinnitus(+), tinnitus(−) and control (ctrl) rat. A rat brain atlas was used to guide ROI placement. The bar graphs indicate the averaged uptake values per group and brain region. (a) Significantly higher manganese uptake was observed in the superficial and deep amygdalae (AMG_S and AMG_D) of the tinnitus(+) group, compared to the control group, as well as in the (b) nucleus accumbens core regions (NA_C). *Indicates statistical significance (p < 0.05). Atlases were adapted from The Rat Brain In Sterotaxic Coordinates, 4^th Edition by Paxinos and Watson (1998) with permission from the publisher.