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. 2017 Jul 7;7:4879. doi: 10.1038/s41598-017-04853-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HpaB and HpaD: two putative type III chaperones, encoded by genes located on the hrp gene cluster, are conserved among all the four phylotypes of the R. solanacearum species complex (RSSC). (a) Schematic representation of the RSSC hrp gene cluster. The arrows represent the different genes. The green, blue, red and yellow color indicates the hpa, prh, hrp and hrc genes, respectively. (b) Protein identity matrix table of HrcV, HpaB and HpaD from nine RSSC sequenced strains belonging to the four phylotypes compared to the GMI1000 (phylotype I) strain. Matrix cells have been colored from orange to yellow according to the protein conservation (100% orange to 70% yellow). Xcv: Xanthomonas campestris pv. vesicatoria. (c) Unrooted phylogenetic tree of HpaD based on sequence similarities using the maximum likelihood method. Ten strains of RSSC were used. Bootstraps were calculated using the approximate likelihood ratio test. The phylogenic tree has been generated using MEGA software.