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. 2017 Jul 7;7:4866. doi: 10.1038/s41598-017-04978-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression profiling of cytokines expressed by CD8⁺ T cells in peripheral blood after HSRT. PBMCs were treated with a leukocyte activation cocktail for 6 h, and the cells were then collected and labeled with IFN-γ (g), TNF-α (g), IL-2 (h), TGF-β (h), IL-1β (i), granzyme B (i), or IL-22 (j). The mean values of the frequencies of TNFα⁺CD8⁺ T cells in CD8⁺ T cells (a), IFNγ⁺CD8⁺ T cells in CD8⁺ T cells (b), TNFα⁺IFNγ⁺CD8⁺ T cells in CD8⁺ T cells (c), IL-2⁺CD8⁺ T cells in CD8⁺ T cells (d), granzyme B⁺ CD8⁺ T cells in CD8⁺ T cells (e), and TGFβ⁺CD8⁺ T cells in CD8⁺ T cells (f) were analyzed at the indicated times. The panel g–j showed the representative result from one patient. The data are shown as the mean values ± SEM (n = 6). Statistics were derived using ANOVA with Bonferroni’s post-test. *p < 0.0167, **p < 0.01; ***p < 0.001.