Table 3.
Identification and characterization of differential expression proteins by MALDI/TOF/TOF MS between acetate or phenol as the sole carbon.
| Identified proteins | Mas scorea | Spot No.b | GI No. | Calculated pI value c | Sequence coverage (%) | Mass (Da)d | Proteins location | Express ion of acetate/acetate plus phenol (fold change) |
|---|---|---|---|---|---|---|---|---|
| Aconitate hydratase B | 169 | 1 | 1310689 | 4.99 | 22 | 95384 | Cytoplasm | ↓ 2.67829 |
| TonB-dependent Receptor Plug domain protein | 136 | 2 | 1310709 | 5.10 | 28 | 36470 | Outer membrane | ↓ 2.01854 |
| Urocanate hydratase OS | 353 | 3 | 470 | 5.63 | 26 | 59646 | Cytoplasm | ↓ 2.73441 |
| 60 kDa chaperonin, groL | 193 | 4 | 903916 | 4.92 | 24 | 57189 | Cytoplasm | ↓ 1.98799 |
| Serine hydroxymethyltransferase, | 173 | 5 | 557600 | 5.44 | 31 | 45195 | Cytoplasm | ↓ 2.10096 |
| 3-oxoadipyl-CoA thiolase | 270 | 6 | 1310697 | 5.89 | 51 | 41279 | Cytoplasm | ↑ 2.18868 |
| Phenol hydroxylase P5 protein | 124 | 7 | 1310911 | 4.53 | 18 | 39500 | Cytoplasm | ↑ Only in acetate + phenol |
| Pirin family protein OS | 147 | 8 | 1310689 | 4.96 | 13 | 35227 | Cytoplasm | ↑ Only in acetate + phenol |
| Muconate and chloromuconate cycloisomerases family protein | 196 | 9 | 1343073 | 5.41 | 27 | 39275 | Cytoplasm | ↑ Only in acetate + phenol |
| Catechol 1,2-dioxygenase, catA | 140 | 10 | 1343073 | 4.70 | 37 | 33526 | Cytoplasm | ↑ 24.5042 |
| MetA-pathway of phenol degradation family protein OS | 81 | 11 | 1358412 | 5.30 | 26 | 32212 | Outer membrane | ↑ 9.57105 |
| 3-oxoadipate CoA-transferase subunit B (Fragment) | 181 | 12 | 1310659 | 4.86 | 42 | 22789 | Cytoplasm | ↑ Only in acetate + phenol |
| 3-oxoadipate CoA-transferase subunit A (Fragment) | 149 | 13 | 1310720 | 7.66 | 46 | 19886 | Cytoplasm | ↑ Only in acetate + phenol |
| Uncharacterized protein | 133 | 14 | 1148157 | 4.85 | 19 | 23098 | Cytoplasm | ↑ 1.81059 |
| Nitroreductase family protein | 118 | 15 | 1310689 | 5.83 | 41 | 21938 | Cytoplasm | ↑ Only in acetate + phenol |
| Fimbrial protein | 205 | 16 | 575584 | 7.77 | 39 | 13897 | Outer membrane | ↑ Only in acetate + phenol |
| OsmC-like family protein | 176 | 17 | 1310689 | 5.71 | 66 | 14449 | Cytoplasm | ↑ Only in acetate + phenol |
| Twitching mobility protein, pilT | 40 | 18 | 1310605 | 6.27 | 23 | 35731 | Cytoplasm | ↑ Only in acetate + phenol |
| Cupin domain protein | 112 | 19 | 1310689 | 5.37 | 25 | 26710 | Cytoplasm | ↑ Only in acetate + phenol |
| Isocitrate lyase | 216 | 20 | 470 | 5.30 | 19 | 59646 | Cytoplasm | ↑ Only in acetate + phenol |
aMOWSE scores of the highest confident matches (P < 0.05).
bThe number refers to the spot numbers as given in Fig. 6.
cpI, the predicted isoelectric point calculated from the protein sequence.
dThe predicted molecular weight calculated from the protein sequence.