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. 2017 Jul 7;7:4892. doi: 10.1038/s41598-017-05290-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PvTAT gene expression level, TAT activity, RA accumulation in wild-type and transgenetic hairy root lines, and the correlation between PvTAT gene expression level, TAT activity, and RA accumulation in hairy root lines. (a) Gene expression was determined by qRT-PCR and is shown relative to the expression level of the housekeeping gene β-actin. Transcripts of PvTAT in the wild-type hairy root lines were set to 1. (b) TAT enzyme activity was measured as describled in the Method. (c) RA accumulation was determined by HPLC. (d) Shows the correlation between PvTAT gene expression level, TAT activity, and RA accumulation in hairy root lines. Data are means ± SD of three replicates. *P < 0.05 and **P < 0.01 as determined by Tukey HSD test. WT, the untransformed hairy roots lines (wild-type control); VC, empty vector transformed hairy roots lines (vector control); Anti-TAT, antisense-expressed TAT hairy root lines; Sense-TAT, sense-expressed TAT hairy root lines.