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. 2017 Jul 7;7:4897. doi: 10.1038/s41598-017-05078-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LMNB1 overexpression increases the proportion of NSC differentiation into neurons. NSCs from C57BL6/J embryos were transfected with pEGFP or pLMNB1-EGFP and differentiated for 2, 4 or 6 days. (A–D) Fluorescence images of immunoreactivity for βIII-tubulin (A,B; red), GFAP (C,D; red) and EGFP (A–D; green) in NSCs differentiated for 4 days. Nuclei are counterstained with DAPI (blue). Scale bars: 50 µm. (E) Quantitative analysis of differentiated cells. Data represent the percentage of neurons, astrocytes, and oligodendrocytes co-expressing EGFP out of the total number of EGFP⁺ cells. *p < 0.05, **p < 0.01 vs pEGFP transfected cells at the respective differentiation time, Student’s t-test. Approximately 400 EGFP⁺ cells were quantified for each staining condition in 3 independent experiments. (F) Quantitative analysis of NSC pyknotic nuclei. 150 cells per condition were counted. In all graphs, bars represent the average ± SEM from 3 independent experiments.