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. 2017 Jul 7;13(8):98. doi: 10.1007/s11306-017-1236-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Changes in extracellular and intracellular glucose concentrations. At 0 h, 500 ng/mL of everolimus was added to the cultured media (EVE). a The cultured media were collected for measurement of extracellular glucose at 48 h. The original (0 h) concentration of cultured media was 4.5 mg/mL. The open bar represents the glucose concentration of the 48 h control sample and the filled bar denotes a sample exposed to everolimus for 48 h. Data represent the mean ± S.D. (n = 3). Statistically significant difference between EVE and control: **p < 0.01 (Student’s t-test). b Intracellular glucose concentration at 48 h. Open bars represent 48 h control cells and filled bars denote 48 h everolimus-exposed cells. Data represent the mean ± S.D. (n = 3). Statistically significant difference between EVE and control: *p < 0.05 (Student’s t-test)