. 2017 May 24;101(14):5709–5721. doi: 10.1007/s00253-017-8334-1

Table 2.

Primer pairs used for the analysis of plasmid-free derivatives of IBB477 and for construction of IBB477 deletion mutants

Plasmid/mutant name	Primers^a
pIBB477a	p477a_F TGCAAATCCTACACATGACACAAT p477a_R GCTTCAACGGCTTCTCCTAA
pIBB477b	p477b1_F GCGGAGCCAAGAGAAGGTA^b p477b1_R CTTAAAAGCATCAAACAAACT
pIBB477b	p477b2_F CAGCCAAGTAATCGTCGCATAA p477b2_R CGAATCCATCAAAGTTTAGGGTAT
pIBB477c	p477c1_F GGCAAACAATCCTGAAAAGTA^b p477c1_R GACATCAGCTTGCCCTACTCG
pIBB477c	p477c2_F TTGGAGATTATCGCTGGTGAACTA p477c2_R TCTAACCGCCAAACAACGAT
pIBB477d	p477d1_F TTATGACAGGGAGGCGTTAG^b p477d1_R CCGACCAATCGATAGCATAG
pIBB477d	p477d2_F CGCAGGAAGAAGTCCAAACC p477d2_R AGATACCTGCACGCTGTGTC
pIBB477e	p477e_F TCCGCTATGTCCATAATCCG^b p477e_R ATTTACGCCACCACTCTAGG
Δ14140	14140-u_F AACCTCTATCGCTCCCTATG 14140-u_R CGAATT-CAATGGTCACTTCCTGATTAGC (EcoRI) 14140-d_F CGAA-TTCACGCTTCCCAATTAGTCAAC (EcoRI) 14140-d_R GGCCATCTTGATTGTTAGGG
ΔprtP	prtP-u_F AACAGTCACATTGGCGAAAG prtP-u_R CGAA-TTCAGCGGAAGCAACTGTGG (EcoRI) prtP-d_F CGAATT-CCGACATTGCTGACACATTG (EcoRI) prtP-d_R GATACGCTGCTGCCCTAAAC

^aRestriction sites are indicated in bold letters

^bPrimer pairs used for multiplex PCR