Table 1.
Overview over cultivations—process variables and outcomes
| FB ID |
q
s gluc (g/(g × h)) |
q
s ara (g/(g × h)) |
q
s total (g/(g × h)) |
x ara (%) |
μ
(1/h) |
Titer (g/l) |
k
(−) |
LE (%) |
Purity hom. (%) |
Purity lysed (%) |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 0.220 | 0.055 | 0.275 | 20.0 | 0.08 | 3.2 | – | – | 70 | – |
| 2 | 0.240 | 0.059 | 0.299 | 19.8 | 0.10 | 2.5 | −.54 | 81.9 | 61 | 53 |
| 3 | 0.263 | 0.143 | 0.406 | 35.1 | 0.12 | 2.1 | −1.25 | 99.0 | 50 | 47 |
| 4 | 0.202 | 0.222 | 0.423 | 52.3 | 0.16 | 1.5 | −.52 | 90.2 | 55 | 41 |
| 5 | 0.119 | 0.051 | 0.179 | 28.4 | 0.05 | 4.0 | −.74 | 75.5 | 58 | 55 |
| 6 | 0.127 | 0.127 | 0.254 | 50.0 | 0.09 | 3.5 | −.47 | 61.7 | 61 | 54 |
| 7 | 0.131 | 0.141 | 0.272 | 51.9 | 0.10 | 3.8 | −.7 | 47.4 | 57 | 60 |
| 8 | 0.135 | 0.227 | 0.362 | 62.7 | 0.12 | 2.5 | −.92 | 75.6 | 49 | 43 |
| 9 | 0.080 | 0.087 | 0.168 | 52.1 | 0.05 | 6.1 | −.97 | 39.3 | 71 | 59 |
| 10 | 0.049 | 0.152 | 0.200 | 75.8 | 0.07 | 5.3 | −0.68 | 31.0 | 79 | 58 |
The cultivations are numbered with a fed-batch ID (FB ID). Values for the mean q s D-glucose (q s gluc), q s L-arabinose (q s ara), q s total, and specific growth rate (μ) during the induction phase are given. Furthermore, the L-arabinose concentration in the mixed feed (x ara) is shown. The rhBMP-2 titer at the end of the induction phase (titer) represents the productivity in each process. Information about E-lysis can be taken from the maximal lysis efficiency (LE) and the lysis reaction coefficient k. The purity is presented for homogenized samples at the end of the induction phase (purity hom.) and an E-lysed non-homogenized sample at maximal E-lysis efficiency (purity lysed)