Fig. 4.

Quantification of apoptotic cells in OVA-sensitized and saline-sensitized skin samples from wild-type, Fas (−) and FasL (−) mice. a Representative images of M30 + cells (red) identified by immunofluorescent method in the in paraffin-embedded slides prepared from the skin of C57BL/6 mice Fas (−) and FasL (−) mice, sensitized with ovalbumin (OVA+). The nuclei were counterstained with Hoechst 33342 (blue). White arrow indicates positive cells. White scale bar shows 100 µm. b Total M30 + cells/field were enumerated in skin samples that included both dermis and epidermis. Values are mean ± SEM from 10 mice. *Significant differences with p ≤ 0.05 in comparison to wild-type mice