. 2017 May 26;54(8):2519–2531. doi: 10.1007/s13197-017-2697-2

Table 2.

Results obtained experimentally and estimated by the models for total yields and total antioxidant activity evaluated by ABTS, DPPH and FRAP assays for each extraction condition

Sample	Total yields (%)		ABTS (mg Trolox Eq./g DW)		DPPH (mg Trolox Eq./g DW)		FRAP (mg AIS Eq./g DW)
Sample	Observed	Predicted (%variation)¹	Observed	Predicted (%variation)¹	Observed	Predicted (%variation)¹	Observed	Predicted (%variation)¹
P0.1/T5/S0	5.38 ± 0.29	5.17 (3.61)	4.22 ± 0.09	4.02 (4.77)	2.41 ± 0.27	2.46 (−2.83)	6.22 ± 0.13	6.05 (2.82)
P0.1/T5/S40	4.54 ± 0.18	4.59 (−1.26)	3.68 ± 0.11	3.83 (−4.08)	2.30 ± 0.13	2.48 (−8.15)	5.47 ± 0.07	5.90 (−7.63)
P0.1/T5/S80	2.84 ± 0.20	3.05 (−7.44)	2.00 ± 0.12	1.89 (5.26)	1.82 ± 0.20	1.63 (9.78)	3.73 ± 0.20	3.48 (6.14)
P0.1/T17.5/S0	5.59 ± 0.13	5.62 (−0.64)	4.32 ± 0.04	4.02 (6.93)	2.50 ± 0.12	2.56 (−2.37)	6.38 ± 0.11	6.24 (2.23)
P0.1/T17.5/S40	5.11 ± 0.06	4.98 (2.48)	3.72 ± 0.23	3.84 (−3.51)	2.44 ± 0.02	2.52 (−3.45)	5.89 ± 0.20	6.07 (−3.10)
P0.1/T17.5/S80	3.46 ± 0.14	3.38 (2.33)	1.80 ± 0.12	1.90 (−5.88)	1.34 ± 0.09	1.62 (−21.37)	3.77 ± 0.23	3.63 (2.68)
P0.1/T30/S0	5.32 ± 0.07	5.49 (−3.36)	3.42 ± 0.12	3.86 (−12.96)	2.56 ± 0.22	2.46 (3.77)	6.19 ± 0.07	6.21 (−0.37)
P0.1/T30/S40	4.36 ± 0.05	4.80 (−10.07)	3.69 ± 0.12	3.68 (0.33)	2.37 ± 0.14	2.37 (−0.22)	5.72 ± 0.12	6.01 (−5.66)
P0.1/T30/S80	3.62 ± 0.25	3.14 (13.21)	1.80 ± 0.07	1.74 (9.42)	1.34 ± 0.29	1.41 (18.38)	3.77 ± 0.13	3.56 (6.92)
P300/T5/S0	5.99 ± 0.12	6.00 (−0.10)	3.59 ± 0.20	4.13 (−15.27)	2.47 ± 0.20	2.39 (2.74)	6.42 ± 0.06	6.44 (−0.25)
P300/T5/S40	5.00 ± 0.16	5.10 (−1.97)	3.80 ± 0.15	3.81 (−0.23)	2.63 ± 0.23	2.40 (8.23)	6.01 ± 0.13	6.17 (−2.62)
P300/T5/S80	3.10 ± 0.26	3.23 (−4.53)	1.64 ± 0.11	1.73 (−6.01)	1.63 ± 0.15	1.54 (4.53)	3.60 ± 0.21	3.65 (−1.50)
P300/T17.5/S0	6.64 ± 0.14	6.48 (2.48)	4.57 ± 0.13	4.23 (7.32)	2.73 ± 0.30	2.59 (4.13)	6.76 ± 0.19	6.63 (2.01)
P300/T17.5/S40	5.48 ± 0.07	5.52 (−0.63)	4.07 ± 0.12	3.91 (3.73)	2.50 ± 0.08	2.55 (−2.14)	6.76 ± 0.22	6.35 (6.11)
P300/T17.5/S80	3.71 ± 0.20	3.59 (3.03)	1.85 ± 0.10	1.84 (0.71)	1.60 ± 0.15	1.64 (−2.87)	3.76 ± 0.12	3.80 (−1.32)
P300/T30/S0	6.61 ± 0.10	6.38 (3.52)	4.38 ± 0.24	4.17 (4.64)	2.51 ± 0.33	2.59 (−4.76)	6.72 ± 0.27	6.60 (1.77)
P300/T30/S40	5.41 ± 0.23	5.36 (0.79)	4.00 ± 0.12	3.86 (3.48)	2.42 ± 0.17	2.50 (−3.54)	6.39 ± 0.11	6.29 (1.38)
P300/T30/S80	3.02 ± 0.06	3.38 (−11.65)	1.60 ± 0.14	1.78 (−11.50)	1.10 ± 0.09	1.53 (−39.64)	3.46 ± 0.27	3.73 (−9.11)
P600/T5/S0	6.37 ± 0.17	6.57 (3.19)	4.45 ± 0.09	4.26 (4.31)	2.65 ± 0.19	2.50 (5.15)	6.54 ± 0.09	6.60 (−1.24)
P600/T5/S40	5.63 ± 0.17	5.34 (5.09)	3.90 ± 0.01	3.80 (2.53)	2.36 ± 0.24	2.51 (−6.71)	6.57 ± 0.11	6.23 (5.16)
P600/T5/S80	3.34 ± 0.17	3.15 (5.39)	1.77 ± 0.13	1.59 (9.93)	1.28 ± 0.13	1.64 (−28.70)	3.55 ± 0.21	3.59 (−1.32)
P600/T17.5/S0	6.75 ± 0.12	7.07 (−4.78)	4.31 ± 0.13	4.47 (−3.71)	2.37 ± 0.16	2.81 (−18.89)	6.51 ± 0.19	6.80 (−4.46)
P600/T17.5/S40	5.75 ± 0.26	5.79 (−0.90)	3.75 ± 0.07	4.01 (−6.98)	2.94 ± 0.26	2.76 (5.80)	6.52 ± 0.26	6.40 (1.74)
P600/T17.5/S80	3.43 ± 0.25	3.54 (-3.50)	1.68 ± 0.10	1.80 (−7.48)	2.30 ± 0.28	1.83 (19.57)	3.53 ± 0.32	3.75 (−6.56)
P600/T30/S0	7.13 ± 0.25	7.00 (1.77)	4.40 ± 0.09	4.51 (−2.45)	3.08 ± 0.23	2.91 (5.05)	6.58 ± 0.08	6.77 (−2.75)
P600/T30/S40	5.82 ± 0.21	5.66 (2.59)	4.23 ± 0.08	4.06 (4.08)	2.77 ± 0.36	2.81 (−2.53)	6.66 ± 0.35	6.35 (4.55)
P600/T30/S80	3.26 ± 0.23	3.35 (−3.11)	1.84 ± 0.09	1.84 (−0.22)	1.84 ± 0.10	1.83 (0.51)	3.71 ± 0.13	3.68 (0.48)
P300/T17.5/S40	5.64 ± 0.15	5.52 (2.12)	3.86 ± 0.02	3.91 (−1.26)	2.63 ± 0.02	2.55 (2.89)	6.27 ± 0.17	6.35 (−1.25)
P300/T17.5/S40	5.55 ± 0.15	5.52 (0.53)	3.80 ± 0.24	3.91 (−3.32)	2.33 ± 0.17	2.55 (−9.88)	6.35 ± 0.24	6.35 (0.01)
P300/T17.5/S40	5.41 ± 0.12	5.52 (−2.10)	4.03 ± 0.11	3.91 (2.86)	2.86 ± 0.22	2.55 (10.40)	6.20 ± 0.13	6.35 (−2.30)

Sample	Total phenolic (mg GA Eq./g DW)		Total tannins (mg catechin Eq./g DW)		Total flavonoids (mg quercetin Eq./g DW)
Sample	Observed	Predicted (%variation)¹	Observed	Predicted (%variation)¹	Observed	Predicted (%variation)¹
P0.1/T5/S0	2.050 ± 0.109	2.054 (−0.42)	0.442 ± 0.026	0.436 (1.00)	0.049 ± 0.007	0.082 (−67.98)
P0.1/T5/S40	1.862 ± 0.116	1.939 (−4.44)	0.396 ± 0.015	0.403 (−1.92)	0.150 ± 0.010	0.140 (6.14)
P0.1/T5/S80	1.084 ± 0.060	1.015 (6.17)	0.415 ± 0.011	0.401 (3.39)	0.101 ± 0.006	0.089 (11.46)
P0.1/T17.5/S0	2.206 ± 0.038	2.199 (0.28)	0.481 ± 0.035	0.469 (2.23)	0.105 ± 0.003	0.095 (43.89)
P0.1/T17.5/S40	1.949 ± 0.039	2.056 (−5.49)	0.420 ± 0.006	0.430 (−2.26)	0.159 ± 0.007	0.155 (−5.04)
P0.1/T17.5/S80	1.106 ± 0.046	1.103 (0.12)	0.414 ± 0.012	0.422 (−2.04)	0.148 ± 0.004	0.105 (0.43)
P0.1/T30/S0	2.231 ± 0.082	2.241 (−0.54)	0.459 ± 0.013	0.482 (−5.04)	0.049 ± 0.004	0.073 (−51.81)
P0.1/T30/S40	2.062 ± 0.034	2.069 (−0.35)	0.429 ± 0.021	0.436 (−1.84)	0.110 ± 0.004	0.135 (−22.66)
P0.1/T30/S80	1.106 ± 0.021	1.089 (10.48)	0.445 ± 0.022	0.422 (−4.95)	0.079 ± 0.004	0.086 (−8.87)
P300/T5/S0	2.306 ± 0.071	2.178 (5.50)	0.479 ± 0.013	0.465 (2.84)	0.059 ± 0.003	0.071 (−22.20)
P300/T5/S40	2.008 ± 0.129	2.036 (−1.67)	0.402 ± 0.015	0.428 (-6.74)	0.140 ± 0.006	0.139 (0.57)
P300/T5/S80	1.049 ± 0.017	1.085 (−3.49)	0.419 ± 0.009	0.424 (−1.10)	0.085 ± 0.004	0.096 (−13.23)
P300/T17.5/S0	2.375 ± 0.047	2.325 (2.05)	0.513 ± 0.011	0.492 (4.07)	0.056 ± 0.004	0.089 (-59.91)
P300/T17.5/S40	2.306 ± 0.129	2.155 (6.34)	0.481 ± 0.011	0.449 (6.55)	0.155 ± 0.006	0.157 (−1.59)
P300/T17.5/S80	1.095 ± 0.048	1.176 (−7.51)	0.441 ± 0.014	0.438 (0.45)	0.109 ± 0.003	0.115 (−5.66)
P300/T30/S0	2.406 ± 0.048	2.369 (1.53)	0.529 ± 0.023	0.498 (5.79)	0.089 ± 0.007	0.071 (19.66)
P300/T30/S40	2.032 ± 0.107	2.171 (−7.03)	0.440 ± 0.030	0.449 (−2.54)	0.139 ± 0.006	0.141 (21.00)
P300/T30/S80	1.081 ± 0.041	1.163 (−7.78)	0.434 ± 0.012	0.432 (0.13)	0.113 ± 0.006	0.100 (10.86)
P600/T5/S0	2.076 ± 0.231	2.230 (−8.28)	0.478 ± 0.025	0.497 (−4.04)	0.091 ± 0.005	0.064 (28.75)
P600/T5/S40	2.125 ± 0.116	2.062 (2.76)	0.476 ± 0.029	0.457 (3.73)	0.136 ± 0.007	0.140 (−3.05)
P600/T5/S80	1.124 ± 0.039	1.084 (3.51)	0.454 ± 0.015	0.449 (0.88)	0.117 ± 0.002	0.105 (10.01)
P600/T17.5/S0	2.397 ± 0.185	2.380 (0.30)	0.499 ± 0.002	0.517 (−3.72)	0.084 ± 0.002	0.086 (−1.65)
P600/T17.5/S40	2.199 ± 0.066	2.183 (0.66)	0.506 ± 0.035	0.472 (6.51)	0.159 ± 0.002	0.162 (−2.27)
P600/T17.5/S80	1.122 ± 0.052	1.177 (−5.11)	0.452 ± 0.006	0.458 (−1.27)	0.111 ± 0.006	0.129 (−16.00)
P600/T30/S0	2.356 ± 0.064	2.426 (−3.01)	0.493 ± 0.020	0.517 (−5.03)	0.059 ± 0.002	0.073 (−23.59)
P600/T30/S40	2.328 ± 0.071	2.201 (5.40)	0.501 ± 0.014	0.465 (6.98)	0.148 ± 0.005	0.150 (−1.99)
P600/T30/S80	1.183 ± 0.071	1.167 (1.12)	0.420 ± 0.022	0.446 (−6.40)	0.123 ± 0.006	0.118 (4.03)
P300/T17.5/S40	2.121 ± 0.129	2.155 (−1.85)	0.436 ± 0.013	0.449 (−3.09)	0.158 ± 0.002	0.157 (0.81)
P300/T17.5/S40	2.148 ± 0.089	2.155 (−0.45)	0.434 ± 0.036	0.449 (−4.04)	0.153 ± 0.004	0.157 (−2.43)
P300/T17.5/S40	2.198 ± 0.160	2.155 (1.59)	0.418 ± 0.022	0.449 (−7.69)	0.156 ± 0.005	0.157 (0.78)

All results were expressed as mg of standard equivalent (Eq.)/g of dried weight (DW)

Mean values ± standard deviation

P, T and S meaning pressure, extraction time and solvent concentration, respectively

¹ $V a r i a t i o n (%) = \frac{o b s e r v e d - p r e d i c t e d}{o b s e r v e d} \times 100$