Figure 1. Size exclusion chromatography was used for further purification of FLRT-FnIII samples.

Initially purification was attempted without DTT, which resulted in monomeric and dimeric forms of FLRT2-FnIII as seen from the SEC curve (green, dash line). After including DTT, the presence of dimeric forms is significantly reduced although a small fraction is still present (green). Subsequent purifications of FLRT1- (blue) and FLRT3-FnIII (red) included DTT, and resulted in similar profile with monomers as the predominant form. Standards in grey (Blue Dextran to determine Void volume, Vo; Conalbumine, 75 kDa; Carbonic Anhydrase, 29 kDa; Aprotenin, 6.5 kDa) were used to calibrate the column, and estimate the molecular weights (Mw) of the FnIII domains.