Figure 2. SDS-PAGE gel following purification and refolding of FLRT1-FnIII together with purified and concentrated FLRT2-FnIII and FLRT3-FnIII domains.

To verify purity and to follow expression, refolding and purification steps, samples were taken at different steps; flow through the nickel-column (FT), wash of immobilized sample (W), application of refolding gradient (G), and elution (E). Also, the SEC purification samples were taken from different fractions (F1 and F2). FLRT2-FnIII and FLRT3-FnIII were purified and refolded following the same protocol. Concentrated samples can be seen next to FLRT1-FnIII purification, showing pure proteins. Markers are also shown (Std).