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. 2017 Jul 7;5:e3550. doi: 10.7717/peerj.3550

Figure 4. Far-UV circular dichroism spectroscopy was performed to assess if proteins were folded.

Figure 4

CD spectroscopy experiments were conducted on refolded FLRT-FnIII domains (FLRT1-FnIII (blue), FLRT2-FnIII (green) and FLRT3-FnIII (red)) in PBS buffer. All samples have a positive signal around ∼230 nm, which is characteristic for fibronectin domains. MRE is mean residue ellipticity.